Product Name	Recombinant Rabbit Anti-PPCS Monoclonal Antibody
Cat No.	SLA2546M
Chinese Name	磷酸泛酰半胱氨酸合成酶重组兔单抗
Alias	CMD2C; 6330579B17Rik; RGD1311399; PPCS_HUMAN; PPCS; Phosphopantothenoylcysteine synthetase (PPC synthetase); 6.3.2.51; COAB; PPCS_MOUSE; phosphopantothenoylcysteine synthetase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3E1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PPCS
Purification	affinity purified by Protein A
Predicted MW.	34 kDa
Observed MW.	34 kDa
Gene ID	79717
Swiss-Prot	Q9HAB8
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.Has a preference for ATP over CTP as a cosubstrate.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PPCS monoclonal antibody, unconjugated (SLA2546M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 7. Paraformaldehyde-fixed, paraffin embedded human small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPCS Monoclonal Antibody, Unconjugated (SLA2546M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.