Product Name	ARG2 | Arginase-2 Recombinant Rabbit mAb
Cat No.	SLM-62356R
Chinese Name	精氨酸酶2重组兔单抗
Alias	AII; ARGI2_HUMAN; ARG2; Arginase II; Kidney-type arginase; Non-hepatic arginase; Type II arginase; 3.5.3.1; ARGI2_MOUSE; ARGI2_RAT; arginase 2; arginase, type II
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	6B9
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ARG2
Purification	affinity purified by Protein A
Concentration
Predicted MW.	39 kDa
Observed MW.	40 kDa
Subcellular Location	N/A
Gene ID	384
Swiss-Prot	P78540
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Arginase2 monoclonal antibody, unconjugated (SLM-62356R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Arginase2 Monoclonal Antibody, Unconjugated (SLM-62356R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.