Product Name	Recombinant Rabbit Anti-TP53INP1 Monoclonal Antibody
Cat No.	SLA1983M
Chinese Name	p53诱导核蛋白1重组兔单抗
Alias	SIP; TP53DINP1; TP53INP1A; TP53INP1B; Teap; p53DINP1; 2700057G22Rik; SIP18; SIP27; Stinp; Tp53inp1; Trp53inp1; T53I1_HUMAN; Stress-induced protein; p53-dependent damage-inducible nuclear protein 1 (p53DINP1); T53I1_MOUSE; Thymus-expressed acidic protein (TEAP); T53I1_RAT; tumor protein p53 inducible nuclear protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5B1
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human p53 DINP1
Purification	affinity purified by Protein A
Predicted MW.	27 kDa
Observed MW.	32 kDa
Gene ID	94241
Swiss-Prot	Q96A56
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with p53 DINP1 monoclonal antibody, unconjugated (SLA1983M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p53 DINP1 Monoclonal Antibody, Unconjugated (SLA1983M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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