Product Name	Recombinant Rabbit Anti-BBC3 Monoclonal Antibody
Cat No.	SLA1302M
Chinese Name	p53正向细胞凋亡调控因子重组兔单抗
Alias	JFY-1; JFY1; PUMA; PUMA/JFY1; BBC3_HUMAN; BBC3; p53 up-regulated modulator of apoptosis; BBC3_MOUSE; BBC3_RAT; BCL2 binding component 3; p53-upregulated modulator of apoptosis
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9G7
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BBC3
Purification	affinity purified by Protein A
Predicted MW.	21 kDa
Observed MW.	25 kDa
Subcellular Location	Cytoplasm
Gene ID	27113
Swiss-Prot	Q9BXH1
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis.Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with BBC3/PUMA monoclonal antibody, unconjugated (SLA1302M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BBC3/PUMA Monoclonal Antibody, Unconjugated (SLA1302M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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