Product Name	Recombinant Rabbit Anti-F2RL1 Monoclonal Antibody
Cat No.	SLA2116M
Chinese Name	蛋白酶激活受体-2重组兔单抗
Alias	GPR11; PAR2; Gpcr11; PAR-2; PAR2_HUMAN; F2RL1; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; Thrombin receptor-like 1; PAR2_MOUSE; PAR2_RAT; F2R like trypsin receptor 1; coagulation factor II (thrombin) receptor-like 1; proteinase-activated receptor-2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	16C12
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PAR2
Purification	affinity purified by Protein A
Predicted MW.	44 kDa
Observed MW.	55 kDa
Subcellular Location	Membrane
Gene ID	2150
Swiss-Prot	P55085
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Receptor for trypsin and trypsin-like enzymes coupled to G proteins that stimulate phosphoinositide hydrolysis. May have a role in the regulation of vascular tone.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PAR-2 monoclonal antibody, unconjugated (SLA2116M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(SLA2116M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(SLA2116M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(SLA2116M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(SLA2116M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(SLA2116M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.