Product Name	Recombinant Rabbit Anti-DDX17 Monoclonal Antibody
Cat No.	SLA2132M
Chinese Name	ATP依赖解旋酶DDX17重组兔单抗
Alias	P72; RH70; 2610007K22Rik; A430025E01Rik; Gm926; DDX17_HUMAN; DDX17; DEAD box protein 17; DEAD box protein p72; DEAD box protein p82; RNA-dependent helicase p72; 3.6.4.13; DDX17_MOUSE; DEAD-box helicase 17; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD); DEAD (Asp-Glu-Ala-Asp) box polypeptide 17; DEAD (Asp-Glu-Ala-Asp) box helicase 17; p72 RNA helicase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human DDX17
Purification	affinity purified by Protein A
Predicted MW.	80 kDa
Observed MW.	80 kDa
Subcellular Location	Nucleus
Gene ID	10521
Swiss-Prot	Q92841
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. Regulates the alternative splicing of exons exhibiting specific features.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with DDX17 monoclonal antibody, unconjugated (SLA2132M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DDX17 Monoclonal Antibody, Unconjugated (SLA2132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-DDX17 antibody (SLA2132M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.