Product Name	Recombinant Rabbit Anti-CDC5L Monoclonal Antibody
Cat No.	SLA1426M
Chinese Name	细胞分裂周期蛋白5样蛋白重组兔单抗
Alias	CDC5L_HUMAN; CDC5L; Cdc5-like protein; Pombe cdc5-related protein; KIAA0432; PCDC5RP; CDC5L_MOUSE; CDC5L_RAT; cell division cycle 5 like; CDC5 (cell division cycle 5, S. pombe, homolog)-like; CDC5 cell division cycle 5-like (S. pombe); cell division cycle 5-like
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7H3
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CDC5L
Purification	affinity purified by Protein A
Predicted MW.	92 kDa
Observed MW.	100 kDa
Gene ID	988
Swiss-Prot	Q99459
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CDC5L monoclonal antibody, unconjugated (SLA1426M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDC5L Monoclonal Antibody, Unconjugated (SLA1426M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.