Product Name	Recombinant Rabbit Anti-pan CaMK-II Monoclonal Antibody
Cat No.	SLA3127M
Chinese Name	泛钙/钙调素依赖蛋白激酶2重组兔单抗
Alias	CAMKA; CaMKIINalpha; CaMKIIalpha; MRD53; MRT63; CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54; CAMKD; CAMK; CAMK-II; CAMKG; MRD59; 2810011D23Rik; 8030469K03Rik; CaMK II; [d]-CaMKII; CaMKII; mKIAA0968; Ck2b; CAMK1; Camki; RATCAMKI; PK2CDD; PKCCD; KCC2D_BOVIN; CAMK2D; CaM kinase II subunit delta; CaMK-II subunit delta; 2.7.11.17; KCC2B_BOVIN; CAMK2B; CaM kinase II subunit beta; CaMK-II subunit beta; KCC2B_HUMAN; KCC2G_HUMAN; CAMK2G; CaM kinase II subunit gamma; CaMK-II subunit gamma; KCC2D_HUMAN; KCC2A_HUMAN; CAMK2A; CaM kinase II subunit alpha; CaMK-II subunit alpha; KIAA0968; KCC2A_MOUSE; KCC2B_MOUSE; KCC2D_MOUSE; Kiaa4163; KCC2G_MOUSE; KCC2B_RAT; KCC2A_RAT; KCC2G_RAT; KCC2D_RAT; calcium/calmodulin dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; CaM-kinase II alpha chain; calcium/calmodulin-dependent protein kinase II alpha-B subunit; CaM kinase II alpha subunit; CaMK-II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase II delta; Calcium/calmodulin dependent protein kinase II gamma; CaM kinase II alpha; CaM kinase II; CaM kinase II beta; CaM kinase II delta; CaM kinase II gamma
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12A1
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CaMK2 alpha/ beta/ gamma/ delta
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	54/56/63/73 kDa
Observed MW.	50-60 kDa
Subcellular Location	Membrane
Gene ID	818
Swiss-Prot	Q9UQM7
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The immunogen is one synthetic peptide derived from human CaMK2 beta/ gamma/ delta. The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with pan CaMKII monoclonal antibody, unconjugated (SLA3127M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with pan CaMKII Monoclonal Antibody, Unconjugated (SLA3127M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-pan CaMKII antibody (SLA3127M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 9. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (pan CaMKII) monoclonal Antibody, unconjugated (SLA3127M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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