Product Name	Recombinant Rabbit Anti-PKC delta&theta Monoclonal Antibody
Cat No.	SLA2821M
Chinese Name	蛋白激酶C-δ/θ重组兔单抗
Alias	ALPS3; CVID9; MAY1; PKCD; nPKC-delta; D14Ertd420e; PKC[d]; PKCdelta; KPCD_HUMAN; PRKCD; Tyrosine-protein kinase PRKCD; 2.7.11.13; KPCD_MOUSE; KPCD_RAT; protein kinase C delta; protein kinase C, delta
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	15C18
React Species	Human,Mouse,Rat
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PKC delta
Purification	affinity purified by Protein A
Predicted MW.	78,82 kDa
Observed MW.	75 kDa
Gene ID	5580
Swiss-Prot	Q05655
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PKC delta/theta monoclonal antibody, unconjugated (SLA2821M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKC delta/theta Monoclonal Antibody, Unconjugated (SLA2821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.