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Product Name Recombinant Rabbit Anti-SCARB2 Monoclonal Antibody Cat No. SLA2160M Chinese Name 清道夫受体B2重组兔单抗 Alias AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2; LIMPII; SR-BII; 9330185J12Rik; LIMP II; MLGP85; SCRB2_HUMAN; SCARB2; 85 kDa lysosomal membrane sialoglycoprotein (LGP85); CD36 antigen-like 2; Lysosome membrane protein II (LIMP II); Scavenger receptor class B member 2; LIMP2; SCRB2_MOUSE; SCRB2_RAT; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II); lysosome membrane protein 2; lysosomal integral membrane protein II Host Species Rabbit Isotype IgG Clonality Monoclonal Clone No. 9F3 React Species Human,Mouse,Rat Applications WB (1:500-2000)
IHC-P (1:200-500)
IHC-F (1:200-500)
IF (1:200-500)
Flow-Cyt (1:50-100)
IP (1:20-50)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.Immunogen A synthesized peptide derived from human SCARB2 Purification affinity purified by Protein A Predicted MW. 54 kDa Observed MW. 80 kDa Gene ID 950 Swiss-Prot Q14108 Storage 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background Acts as a lysosomal receptor for glucosylceramidase (GBA1) targeting. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Validation Images
Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SCARB2 monoclonal antibody, unconjugated (SLA2160M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCARB2 Monoclonal Antibody, Unconjugated (SLA2160M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.
+86 571 56623320
[email protected]
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