Product Name	Recombinant Rabbit Anti-SNAP25 Monoclonal Antibody
Cat No.	SLA2847M
Chinese Name	突触相关蛋白25重组兔单抗
Alias	CMS18; DEE117; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP; bA416N4.2; dJ1068F16.2; Bdr; GENA70; sp; SNAP-25B; SNAP-25a; SNP25_HUMAN; SNAP25; Super protein (SUP); Synaptosomal-associated 25 kDa protein; SNP25_MOUSE; SNP25_RAT; synaptosome associated protein 25; synaptosomal-associated protein, 25kDa; resistance to inhibitors of cholinesterase 4 homolog
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8E5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human SNAP25
Purification	affinity purified by Protein A
Predicted MW.	23 kDa
Observed MW.	25 kDa
Subcellular Location	Cytoplasm
Gene ID	6616
Swiss-Prot	P60880
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SNAP25 monoclonal antibody, unconjugated (SLA2847M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SNAP25 Monoclonal Antibody, Unconjugated (SLA2847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.