Product Name	Recombinant Rabbit Anti-Aif1 Monoclonal Antibody
Cat No.	SLA1026M
Chinese Name	离子钙接头蛋白单克隆抗体
Alias	AIF-1; IBA1; IRT-1; IRT1; D17H6S50E; G1; BART-1; Bart1; mrf-1; AIF1_HUMAN; AIF1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1_MOUSE; AIF1_RAT; Microglia response factor; Mrf1; allograft inflammatory factor 1; interferon gamma responsive transcript
Host Species	Mouse
Isotype	IgG1, k
Clonality	Monoclonal
Clone No.	10C8
React Species	Human,Mouse,Rat
Applications	IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse Iba1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	16 kDa
Subcellular Location	Cytoplasm
Gene ID	11629
Swiss-Prot	O70200
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Aif1 | Iba1 Monoclonal Antibody, Unconjugated (SLA1026M) at 1:800 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.