Product Name	Recombinant Rabbit Anti-BNIP3 Monoclonal Antibody
Cat No.	SLA1513M
Chinese Name	促凋亡调节蛋白BNIP3重组兔单抗
Alias	HABON; NIP3; BNIP3_HUMAN; BNIP3; BNIP3_MOUSE; BCL2 interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; nineteen kD interacting protein-3; Hypoxia-Activated BNIP3 Overlapping Non-coding RNA
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10C11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-1000) IHC-F (1:100-1000) IF (1:100-1000) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BNIP3
Purification	affinity purified by Protein A
Predicted MW.	22 kDa
Observed MW.	25-28 kDa
Subcellular Location	Cytoplasm
Gene ID	664
Swiss-Prot	Q12983
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Hela (H) cells were treated with or without cobalt chloride (100 μM,overnight), 25 μg total protein per lane of cell lysates (see on figure) probed with BNIP3 monoclonal antibody, unconjugated (SLA1513M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(SLA1513M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.