Product Name	Recombinant Rabbit Anti-CDK1 Monoclonal Antibody
Cat No.	SLA1308M
Chinese Name	磷酸化周期素依赖性激酶1重组兔单抗
Alias	CDC2; CDC28A; P34CDC2; Cdc2a; p34; CDK1_CHICK; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; 2.7.11.22; 2.7.11.23; CDK1_HUMAN; CDKN1; CDK1_MOUSE; CDK1_RAT; cyclin dependent kinase 1; cell division cycle 2, G1 to S and G2 to M; CDK1/2 (phospho-T14); p-CDK1/2; phospho-CDK1/2; CDK1/2 (phospho-Thr14)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	19D6
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CDK1 around the phosphorylation site of T14
Purification	affinity purified by Protein A
Predicted MW.	34 kDa
Observed MW.	34 kDa
Subcellular Location	Nucleus
Gene ID	983
Swiss-Prot	P06493
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Phospho-CDK1/2 (Thr14) monoclonal antibody, unconjugated (SLA1308M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1/2 (Thr14) Monoclonal Antibody, Unconjugated(SLA1308M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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