Product Name	GLUD1 Recombinant Rabbit mAb
Cat No.	SLM-62249R
Chinese Name	谷氨酸脱氢酶1重组兔单抗
Alias	DHE3_HUMAN; GLUD1; GDH 1; 1.4.1.3; GLUD; GDH; GDH1; hGDH1; glutamate dehydrogenase 1
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	3B2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Glutamate dehydrogenase 1
Purification	affinity purified by Protein A
Concentration
Predicted MW.	61 kDa
Observed MW.	55 kDa
Subcellular Location	N/A
Gene ID	2746
Swiss-Prot	P00367
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Glutamate dehydrogenase 1 monoclonal antibody, unconjugated (SLM-62249R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 13. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glutamate dehydrogenase 1 Monoclonal Antibody, Unconjugated (SLM-62249R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 14. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Glutamate dehydrogenase 1) monoclonal Antibody, unconjugated (SLM-62249R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.