Product Name	Recombinant Rabbit Anti-ILF3 Monoclonal Antibody
Cat No.	SLA1915M
Chinese Name	白细胞介素增强子结合因子3重组兔单抗
Alias	CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NF90c; NF90ctv; NFAR; NFAR-1; NFAR-2; NFAR110; NFAR2; NFAR90; TCP110; TCP80; MBII-26; ilf3; wu:fb37d07; wu:fb94b02; zgc:77030; ILF3_HUMAN; Double-stranded RNA-binding protein 76 (DRBP76); M-phase phosphoprotein 4 (MPP4); Nuclear factor associated with dsRNA (NFAR); Nuclear factor of activated T-cells 90 kDa (NF-AT-90); Translational control protein 80 (TCP80); ILF3_MOUSE; ILF3_RAT; ILF3_DANRE; interleukin enhancer binding factor 3; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; M-phase phosphoprotein 4; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells 110 kDa; translational control protein 80; double-stranded RNA-binding protein, 76 kD; M phase phosphoprotein 4, nuclear factor associated with DS RNA; dsRNA binding protein NFAR-2/MPP4
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12B4
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ILF3
Purification	affinity purified by Protein A
Predicted MW.	95 kDa
Observed MW.	85-110 kDa
Subcellular Location	Nucleus
Gene ID	3609
Swiss-Prot	Q12906
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ILF3 monoclonal antibody, unconjugated (SLA1915M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 13. Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 14. Paraformaldehyde-fixed, paraffin embedded Human Esophageal; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 15. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (SLA1915M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 16. The A673 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ILF3 antibody (SLA1915M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.