Product Name	Rabbit Anti-ACTN1 Polyclonal Antibody
Cat No.	SLA0123
Chinese Name	α-辅肌动蛋白1抗体
Alias	BDPLT15; 3110023F10Rik; Actn1a; ACTN1_HUMAN; ACTN1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN1_MOUSE; ACTN1_RAT; actinin alpha 1
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Alpha-actinin-1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	103 kDa
Observed MW.	103 kDa
Subcellular Location	Cytoplasm
Gene ID	87
Swiss-Prot	P12814
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Alpha-actinin-1 polyclonal antibody, unconjugated (SLA0123) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.