Product Name	Recombinant Rabbit Anti-ATG3 Monoclonal Antibody
Cat No.	SLA1545M
Chinese Name	自噬相关蛋白3重组兔单抗
Alias	APG3; APG3-LIKE; APG3L; PC3-96; hApg3; 2610016C12Rik; Atg3l; PIG-1; Pig1; ATG3_HUMAN; ATG3; Autophagy-related protein 3 (APG3-like | hApg3); Protein PC3-96; 2.3.2.-; ATG3_MOUSE; Autophagy-related protein 3 (APG3-like); ATG3_RAT; Preconditioning-inducible gene 1 protein; autophagy related 3; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy related 3 homolog (S. cerevisiae)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9B3
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ATG3
Purification	affinity purified by Protein A
Predicted MW.	36 kDa
Observed MW.	37 kDa
Subcellular Location	Cytoplasm
Gene ID	64422
Swiss-Prot	Q9NT62
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ATG3 monoclonal antibody, unconjugated (SLA1545M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.