Product Name	eIF4A1 Mouse mAb
Cat No.	SLM-33142M
Chinese Name	eIF4A1蛋白单克隆抗体
Alias	IF4A1_BOVIN; EIF4A1; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; 3.6.4.13; IF4A1_HUMAN; DDX2A; EIF4A; IF4A1_MOUSE; eukaryotic translation initiation factor 4A1; eukaryotic translation initiation factor 4A, isoform 1
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	1B8
React Species	Human,Mouse,Rat
Applications	WB (1:500-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human eIF4A1
Purification	affinity purified by Protein G
Concentration	1mg/ml
Predicted MW.	46 kDa
Observed MW.	46 kDa
Subcellular Location	Cytoplasm
Gene ID	1973
Swiss-Prot	P60842
Storage	Size : 50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Mouse Raw264.7 cell lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Human HepG2 cell lysates Lane 4: Human K562 cell lysates Lane 5: Human PANC-1 cell lysates Primary: Anti-eIF4A1 (SLM-33142M) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 46 kDa Observed band size: 46 kDa

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.