Product Name	Rabbit Anti-GRAP Polyclonal Antibody
Cat No.	SLA0017
Chinese Name	生长因子受体结合蛋白2相关接头蛋白抗体
Alias	DFNB114; 8430435N19Rik; GRAP_HUMAN; GRAP; GRAP_MOUSE; GRB2 related adaptor protein
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human GRAP
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	25 kDa
Observed MW.	25 kDa
Subcellular Location	Cytoplasm
Gene ID	10750
Swiss-Prot	Q13588
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences. For instance, the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains. Members include GRB2, GRB7, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein). While GRB10 and GRB14 are most closely related to GRB7, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2. The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, c-Kit and the erythropoietin receptor (EpoR). Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Mouse Prostate tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human A549 cell lysates Lane 5: Human MCF-7 cell lysates Lane 6: Human HeLa cell lysates Primary: Anti-GRAP (SLA0017) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.