Product Name	MRPL15 | L15mt Rabbit pAb
Cat No.	SL17771R
Chinese Name	线粒体核糖体蛋白L15抗体
Alias	HSPC145; L15mt; MRP-L15; MRP-L7; RPML7; uL15m; zgc:92856; RM15_HUMAN; MRPL15; 39S ribosomal protein L15, mitochondrial (L15mt | MRP-L15); RM15_MOUSE; RM15_DANRE; mitochondrial ribosomal protein L15
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human MRPL15
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	31 kDa
Subcellular Location	Cytoplasm
Gene ID	29088
Swiss-Prot	Q9P015
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MRPL15) polyclonal Antibody, unconjugated (SL17771R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.