Product Name	Rabbit Anti-ELANE Polyclonal Antibody
Cat No.	SLA0206
Chinese Name	中性粒细胞弹性蛋白酶ELANE抗体
Alias	ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; F430011M15Rik; ELNE_HUMAN; ELANE; Bone marrow serine protease; Elastase-2; Human leukocyte elastase (HLE); Medullasin; PMN elastase; 3.4.21.37; ELNE_MOUSE; Leukocyte elastase; elastase, neutrophil expressed; elastase 2, neutrophil; neutrophil elastase; polymorphonuclear leukocyte elastase
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Neutrophil Elastase protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	26 kDa
Observed MW.	28 kDa
Subcellular Location	Cytoplasm
Gene ID	1991
Swiss-Prot	P08246
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Neutrophil Elastase polyclonal antibody, unconjugated (SLA0206) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.