Product Name	Recombinant Rabbit Anti-Ocln Monoclonal Antibody
Cat No.	SLA1196M
Chinese Name	紧密连接蛋白重组兔单抗
Alias	Ocl; BLCPMG; PPP1R115; PTORCH1; OCLN_CANLF; OCLN; OCLN_HUMAN; OCLN_MOUSE; OCLN_RAT; occludin; tight junction protein occludin TM4 minus; phosphatase 1, regulatory subunit 115
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3C7
React Species	Mouse,Rat
Applications	WB (1:500-2000) Flow-Cyt (1:100-200) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Occludin
Purification	affinity purified by Protein A
Predicted MW.	57 kDa
Observed MW.	65 kDa
Subcellular Location	Membrane
Gene ID	100506658
Swiss-Prot	Q16625
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Occludin monoclonal antibody, unconjugated (SLA1196M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.