Product Name	Recombinant Rabbit Anti-TJP1 Monoclonal Antibody
Cat No.	SLA0504M
Chinese Name	胞质紧密粘连蛋白1/闭锁小带蛋白1单克隆抗体
Alias	ZO-1; ZO1; ZO1_HUMAN; TJP1; Tight junction protein ZO-1; Zona occludens protein 1; Zonula occludens protein 1; ZO1_MOUSE; tight junction protein 1; zona occludens 1
Host Species	Mouse
Clonality	Monoclonal
Clone No.	12A7
React Species	Human
Applications	WB (1:1000-5000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human ZO-1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	191 kDa
Observed MW.	245 kDa
Subcellular Location	Cytoplasm
Gene ID	7082
Swiss-Prot	Q07157
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (SLA0504M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.