Product Name	Recombinant Rabbit Anti-CBX3 Monoclonal Antibody
Cat No.	SLA0736M
Chinese Name	异染色质蛋白1-γ/HP-1γ重组兔单抗
Alias	HECH; HP1-GAMMA; HP1Hs-gamma; HP1gamma; HP1g; M32; CBX3_HUMAN; CBX3; Heterochromatin protein 1 homolog gamma (HP1 gamma); Modifier 2 protein; CBX3_MOUSE; chromobox 3; chromobox homolog 3 (Drosophila HP1 gamma); chromobox homolog 3; HP1 gamma homolog (Drosophila)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4H6
React Species	Human
Applications	WB (1:500-2000) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human HP1 gamma Protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	21 kDa
Observed MW.	22 kDa
Subcellular Location	Nucleus
Gene ID	11335
Swiss-Prot	Q13185
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CBX3 | HP1 gamma monoclonal antibody, unconjugated (SLA0736M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Figure 2. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HP1 gamma) monoclonal Antibody, unconjugated (SLA0736M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.