Product Name	Rabbit Anti-TREM1 Polyclonal Antibody
Cat No.	SLA0200
Chinese Name	髓系细胞触发受体1抗体
Alias	CD354; TREM-1; TREM1_HUMAN; TREM1; Triggering receptor expressed on monocytes 1; TREM1_MOUSE; triggering receptor expressed on myeloid cells 1
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human TREM1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	24 kDa
Observed MW.
Subcellular Location	Membrane
Gene ID	54210
Swiss-Prot	Q9NP99
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-REM1 antibody (SLA0200): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed Figure 2. 4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (REM1) polyclonal Antibody, unconjugated (SLA0200) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.