Product Name	Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody
Cat No.	SLA2908M
Chinese Name	胚胎干细胞关键蛋白重组兔单抗
Alias	OCT3; OCT4; OCT4Borf1; OTF-3; OTF3; OTF4; Oct-3; Oct-4; Oct3/4; NF-A3; Oct-3/4; Otf-4; Otf3-rs7; Otf3g; PO5F1_HUMAN; POU5F1; Octamer-binding protein 3 (Oct-3); Octamer-binding protein 4 (Oct-4); Octamer-binding transcription factor 3 (OTF-3); PO5F1_MOUSE; POU class 5 homeobox 1; POU domain class 5, transcription factor 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6B9
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Oct 4
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	39 kDa
Observed MW.	42 kDa
Subcellular Location	Nucleus
Gene ID	5460
Swiss-Prot	Q01860
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Oct4 monoclonal antibody, unconjugated (SLA2908M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min Figure 2. The NCCIT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Oct4 antibody (SLA2908M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.