Product Name	PGR | PR Recombinant Rabbit mAb
Cat No.	SLM-60804R
Chinese Name	孕激素受体重组兔单克隆抗体
Alias	NR3C3; PR; 9930019P03Rik; PR-A; PR-B; PRGR_HUMAN; PGR; Nuclear receptor subfamily 3 group C member 3; PRGR_MOUSE; PRGR_RABIT; PRGR_RAT; progesterone receptor
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	9A7
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Progesterone receptor
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	103 kDa
Observed MW.	110 kDa
Subcellular Location	Nucleus
Gene ID	5241
Swiss-Prot	P06401
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-Aand hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Progesterone Receptor monoclonal antibody, unconjugated (SLM-60804R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min

Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Progesterone Receptor Monoclonal Antibody, Unconjugated(SLM-60804R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining

Figure 3. The T-47D (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Progesterone Receptor antibody (SLM-60804R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

Figure 4. 4% Paraformaldehyde-fixed T-47D (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Progesterone Receptor) monoclonal Antibody, unconjugated (SLM-60804R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.