Product Name	Rabbit Anti-PPARGC1A Polyclonal Antibody
Cat No.	SLA0188
Chinese Name	过氧化物酶体增殖物激活受体γ辅激活子1α抗体
Alias	AVPGC-1ALPHA; PPARGC-1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1; A830037N07Rik; Gm11133; PGC-1; PPARGC-1-alpha; Pgco1; LRPGC1; PGCvf; PGCvf-1; PGCvf1; PRGC1_BOVIN; PPARGC1A; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PRGC1_HUMAN; Ligand effect modulator 6; PRGC1_MOUSE; PRGC1_PIG; PRGC1_RAT; PPARG coactivator 1 alpha; peroxisome proliferative activated receptor, gamma, coactivator 1; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PPARgamma coactivator 1alpha
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human PPARGC1A
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	91 kDa
Observed MW.	61 kDa
Subcellular Location	Nucleus
Gene ID	10891
Swiss-Prot	Q9UBK2
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human PPARGC1A, N-Trx-His(SL42280P) Primary: Anti-PPARGC1A (SLA0188) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 61 kDa

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (SLA0188) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (PPARGC1A) Polyclonal Antibody, Unconjugated (SLA0188) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 4. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (SLA0188) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.