Product Name	Recombinant Rabbit Anti-PIK3R4 Monoclonal Antibody
Cat No.	SLA2909M
Chinese Name	磷酸肌肽3激酶调控亚基4重组兔单抗
Alias	PI3R4_HUMAN; PIK3R4; PI3-kinase regulatory subunit 4; PI3-kinase p150 subunit; Phosphoinositide 3-kinase adaptor protein; 2.7.11.1; VPS15; p150; phosphoinositide-3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3G19
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PI3 kinase p150
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	153 kDa
Observed MW.	155 kDa
Gene ID	30849
Swiss-Prot	Q99570
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PI3 kinase p150 monoclonal antibody, unconjugated (SLA2909M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(SLA2909M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(SLA2909M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(SLA2909M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(SLA2909M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(SLA2909M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.