Product Name	Recombinant Rabbit Anti-LTF Monoclonal Antibody
Cat No.	SLA2767M
Chinese Name	乳铁蛋白重组兔单抗
Alias	GIG12; HEL110; HLF2; LF; Csp82; MMS10R; Ms10r; TRFL_HUMAN; LTF; Lactoferrin; Growth-inhibiting protein 12; Talalactoferrin; 3.4.21.-; TRFL_MOUSE; lactotransferrin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	19C8
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Lactoferrin
Purification	affinity purified by Protein A
Predicted MW.	78 kDa
Observed MW.	78 kDa
Subcellular Location	Nucleus
Gene ID	4057
Swiss-Prot	P02788
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Lactoferrin monoclonal antibody, unconjugated (SLA2767M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Lactoferrin Monoclonal Antibody, Unconjugated (SLA2767M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Lactoferrin Monoclonal Antibody, Unconjugated (SLA2767M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Lactoferrin Monoclonal Antibody, Unconjugated (SLA2767M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Lactoferrin Monoclonal Antibody, Unconjugated (SLA2767M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Lactoferrin was immunoprecipitated from human milk lysate with SLA2767M at 1/100 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti-Rabbit IgG (H&L) was used as secondary antibody. Lane 1: human milk lysate (Input). Lane 2: SLA2767M IP in human milk lysate. Lane 3: native rabbit IgG IP in human milk lysate (negative control).

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.