Product Name	Recombinant Rabbit Anti-MBP Monoclonal Antibody
Cat No.	SLA1462M
Chinese Name	髓鞘碱性蛋白/磷脂碱性蛋白重组兔单抗
Alias	Hmbpr; golli-mbp; jve; mld; shi; Mbps; MBP_HUMAN; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein; MBP_MOUSE; MBP_PIG; MBP_RABIT; Myelin P1 protein; MBP_RAT; myelin basic protein
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8D5
React Species	Human,Mouse,Rat
Applications	WB (1:1000-5000) IHC-P (1:200-500) IHC-F (1:200-500) IF (1:200-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Myelin basic protein
Purification	affinity purified by Protein A
Predicted MW.	33 kDa
Observed MW.	46318 kDa
Subcellular Location	Cytoplasm
Gene ID	4155
Swiss-Prot	P02686
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with MBP monoclonal antibody, unconjugated (SLA1462M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:300 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:300 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Monoclonal Antibody, Unconjugated(SLA1462M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.