Product Name	Recombinant Rabbit Anti-PECAM1 Monoclonal Antibody
Cat No.	SLA0607M
Chinese Name	血小板内皮细胞黏附分子-1单克隆抗体
Alias	CD31; CD31/EndoCAM; GPIIA'; PECA1; PECAM-1; endoCAM; Pecam; PECA1_HUMAN; PECAM1; PECA1_MOUSE; platelet and endothelial cell adhesion molecule 1; platelet/endothelial cell adhesion molecule 1; CD31 antigen
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	1F8
React Species	Human
Applications	IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Flow-Cyt (1ug/Test) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD31 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	78 kDa
Observed MW.
Subcellular Location	Membrane
Gene ID	5175
Swiss-Prot	P16284
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (SLA0607M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 7. The EA.hy926(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD31 antibody (SLA0607M): 1 μg/10^6 cells; isotype control (orange): Mouse IgG: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 8. 4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD31) monoclonal Antibody, unconjugated (SLA0607M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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