Product Name	Recombinant Rabbit Anti-Itgax Monoclonal Antibody
Cat No.	SLA0962M
Chinese Name	整合素αX重组兔单抗
Alias	CD11C; SLEB6; Cr4; N418; RGD1561123; ITAX_HUMAN; ITGAX; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; ITAX_MOUSE; integrin subunit alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); complement component 3 receptor 4 subunit
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11G3
React Species	Mouse
Applications	WB (1:500-2000) IHC-P (1:500-2000) IHC-F (1:500-2000) IF (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse CD11c protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	129 kDa
Observed MW.	145 kDa
Subcellular Location	Membrane
Gene ID	16411
Swiss-Prot	Q9QXH4
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD11c monoclonal antibody, unconjugated (SLA0962M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (SLA0962M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.