Product Name	Recombinant Rabbit Anti-ZBTB7A Monoclonal Antibody
Cat No.	SLA2137M
Chinese Name	扑克蒙蛋白重组兔单抗
Alias	FBI-1; FBI1; LRF; MNDLFH; TIP21; ZBTB7; ZNF857A; pokemon; 9030619K07Rik; 9130006G12Rik; ZBT7A_HUMAN; ZBTB7A; Factor binding IST protein 1 (FBI-1); Factor that binds to inducer of short transcripts protein 1; HIV-1 1st-binding protein 1; Leukemia/lymphoma-related factor; POZ and Krueppel erythroid myeloid ontogenic factor (POK erythroid myeloid ontogenic factor | Pokemon | Pokemon 1); TTF-I-interacting peptide 21 (TIP21); Zinc finger protein 857A; ZBT7A_MOUSE; POZ and Krueppel erythroid myeloid ontogenic factor (POK erythroid myeloid ontogenic factor | Pokemon); zinc finger and BTB domain containing 7A; zinc finger and BTB domain containing 7; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; lymphoma related factor; POK erythroid myeloid ontogenic factor; leukemia/lymphoma related factor
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2B1
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Flow-Cyt (1:50-100) ICC/IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ZBTB7A
Purification	affinity purified by Protein A
Predicted MW.	61 kDa
Observed MW.	73 kDa
Subcellular Location	Nucleus
Gene ID	51341
Swiss-Prot	O95365
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon/ZBTB7A) monoclonal Antibody, unconjugated (SLA2137M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with Pokemon/ZBTB7A monoclonal antibody, unconjugated (SLA2137M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(SLA2137M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(SLA2137M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(SLA2137M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(SLA2137M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(SLA2137M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Pokemon/ZBTB7A antibody (SLA2137M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 9. 4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pokemon/ZBTB7A) monoclonal Antibody, unconjugated (SLA2137M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.