Product Name	Recombinant Rabbit Anti-CHUK Monoclonal Antibody
Cat No.	SLA1395M
Chinese Name	KB抑制蛋白激酶α重组兔单抗
Alias	BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16; IKKA_HUMAN; CHUK; I-kappa-B kinase alpha; IKK-A; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1 (IKK-1 | IKK1); Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA); Transcription factor 16 (TCF-16); 2.7.11.10; IKKA_MOUSE; I-kappa-B kinase 1 (IKK1); component of inhibitor of nuclear factor kappa B kinase complex; inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	18F3
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human IKK alpha
Purification	affinity purified by Protein A
Predicted MW.	85 kDa
Observed MW.	80 kDa
Subcellular Location	Nucleus
Gene ID	1147
Swiss-Prot	O15111
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with IKK alpha monoclonal antibody, unconjugated (SLA1395M) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IKK alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IKK alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHUK | IKK-alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHUK | IKK-alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHUK | IKK-alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHUK | IKK-alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHUK | IKK-alpha Monoclonal Antibody, Unconjugated (SLA1395M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining
Figure 9. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IKK alpha antibody (SLA1395M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.