Product Name	Recombinant Rabbit Anti-ATG5 Monoclonal Antibody
Cat No.	SLA0853M
Chinese Name	自噬蛋白5重组兔单抗
Alias	APG5; APG5-LIKE; APG5L; ASP; SCAR25; hAPG5; 2010107M05Rik; 3110067M24Rik; Atg5l; Paddy; ATG5_HUMAN; ATG5; Apoptosis-specific protein; ATG5_MOUSE; ATG5_RAT; autophagy related 5; APG5 (autophagy 5, S. cerevisiae)-like; APG5 autophagy 5-like (S. cerevisiae); ATG5 autophagy related 5 homolog (S. cerevisiae)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4G5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:20-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ATG5
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	32 kDa
Observed MW.	55 kDa
Subcellular Location	Cytoplasm
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ATG5 monoclonal antibody, unconjugated (SLA0853M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG5 Monoclonal Antibody, Unconjugated(SLA0853M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG5 Monoclonal Antibody, Unconjugated(SLA0853M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG5 Monoclonal Antibody, Unconjugated(SLA0853M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG5 | ASP Monoclonal Antibody, Unconjugated (SLA0853M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG5 | ASP Monoclonal Antibody, Unconjugated (SLA0853M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG5 | ASP Monoclonal Antibody, Unconjugated (SLA0853M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG5 | ASP Monoclonal Antibody, Unconjugated (SLA0853M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATG5 | ASP Monoclonal Antibody, Unconjugated (SLA0853M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
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