Product Name	Recombinant Rabbit Anti-MAPT Monoclonal Antibody
Cat No.	SLA0518M
Chinese Name	微管相关蛋白MAPT/Tau单克隆抗体
Alias	tau; DDPAC; FTD1; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; Tau-PHF6; tau-40; Mtapt; PHF-tau; MAPT_0N4R; MAPT_1N4R; MAPT_2N4R; RNPTAU; pTau; TAU_BOVIN; MAPT; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); TAU_HUMAN; TAU_MOUSE; TAU_RAT; microtubule associated protein tau; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; Tau-derived paired helical filament hexapeptide
Host Species	Mouse
Isotype	IgG3
Clonality	Monoclonal
Clone No.	6E2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant Human Tau-4 Protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	46/79 kDa
Observed MW.	50-70 kDa
Subcellular Location	Cytoplasm
Gene ID	4137
Swiss-Prot	P10636-8
Storage	Size : 50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Tau proteins are important Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau proteins subcellular located in the axons of neurons, in the cytoso l and in association with plasma membrane components. It expressed in neurons. PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Tau-4 monoclonal antibody, unconjugated (SLA0518M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(SLA0518M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(SLA0518M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(SLA0518M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated(SLA0518M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of SLA0518M) at 1:1500 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of SLA0518M) at 1:1500 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of SLA0518M) at 1:1500 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (SLA0518M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (SLA0518M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (SLA0518M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.