Product Name	Recombinant Rabbit Anti-Lrp2 Monoclonal Antibody
Cat No.	SLA0546M
Chinese Name	糖蛋白gp330重组兔单抗
Alias	DBS; GP330; LRP-2; D230004K18Rik; Megalin; b2b1625.2Clo; LRP2_HUMAN; LRP2; Glycoprotein 330 (gp330); LRP2_MOUSE; LRP2_RAT; LDL receptor related protein 2; low density lipoprotein receptor-related protein 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9C14
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse LRP2 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	519 kDa
Observed MW.	26 kDa
Subcellular Location	Membrane
Gene ID	14725
Swiss-Prot	A2ARV4
Storage	Size : 25ul/50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Lrp2 | Megalin/gp330 Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Lrp2 | Megalin/gp330 Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Lrp2 | Megalin/gp330 Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 4. Sample: Lane 1: Recombinant mouse LRP2 protein, N-His(SL42103P) Primary: Anti-LRP2 (SLA0546M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 519 kDa Observed band size: 26 kDa
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 9. (Negative control)Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 10. (Negative control)Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 11. (Negative control)Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRP2 Monoclonal Antibody, Unconjugated(SLA0546M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with LRP2 Monoclonal Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C, followed by a Cy3 conjugated Goat Anti-Rabbit IgG antibody (SL0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.
Figure 13. Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with LRP2 Monoclonal Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C, followed by a Cy3 conjugated Goat Anti-Rabbit IgG antibody (SL0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.	Figure 14. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with LRP2 Monoclonal Antibody, Unconjugated (SLA0546M) at 1:200 overnight at 4°C, followed by a Cy3 conjugated Goat Anti-Rabbit IgG antibody (SL0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.