Product overview

Canine Anti-Thyroid-Peroxidase (Anti-TPO) ELISA Kit (Catalog No. SL0309Ca) is an enzyme-linked immunosorbent assay kit for detection of the target in serum, plasma and cell culture supernatant.

Assay principle

The quantitative detection of Canine Anti-TPO is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antigen specific to Canine Anti-TPO. The corresponding antibody in the sample or standards binds the immobilized antigen after incubation. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antigen conjugate is added, which binds to the antibody, forming antigen-antibody-enzyme-labeled antigen complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antibody present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to a cut-off value. Reagent

Specifications

• Assay range: 5.6pg/ml-400pg/ml
• Sensitivity: 1.2pg/ml
• Standard: 540pg/ml

Kit components

• Sealed bags
• Standard (540pg/ml)
• Standard diluent
• HRP-conjugate reagent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)