Summary:

Purpose

Our Quick Step Chicken keyhole limpet hemocyanin antibody IgG(KLH-IgG)ELISA Kit is to for the qualitative determination of KLH-IgG in Chicken serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to KLH-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for KLH-IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain KLH-IgG and HRP conjugated KLH antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of KLH-IgG is determined by comparing with the CUT OFF value.

Determine the result

Test effectiveness: the average value of positive control ≥1.00; The average value

of negative control ≤0.10.

The critical value (CUT OFF) calculation: critical value = the average value of

negative control + 0.15

Negative judgement: if the OD value<CUT OFF, the sample is Chicken KLH-IgG negative.

Positive judgement: if the OD value ≥CUT OFF, the sample is Chicken KLH-IgG positive.