Summary:

Purpose

Our Quick Step Canine Brucella antibody(Brucella-Ab)ELISA Kit is to for the qualitative determination of Brucella-Ab in Canine serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific toBrucella-Ab, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Brucella-Ab is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Brucella-Ab and HRP conjugated Brucella antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Brucella-Ab is determined by comparing with the CUT OFF value.

Determine the result

Test effectiveness: the average value of positive control ≥1.00; The average value

of negative control ≤0.10.

The critical value (CUT OFF) calculation: critical value = the average value of

negative control + 0.15

Negative judgement: if the OD value<CUT OFF, the sample is Canine Brucella-Ab negative.

Positive judgement: if the OD value ≥CUT OFF, the sample is Canine Brucella-Ab positive.