Summary:

Purpose

Our Quick Step Fish Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit is to assay G6PD levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to G6PD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for G6PD is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain G6PD and HRP conjugated G6PD antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of G6PD. You can calculate the concentration of G6PD in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0081FI is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.040	0.0450
Blank Well	0ng/ml	0.050	0.0450
S1	4.8ng/ml	1.958	1.9555
S1	4.8ng/ml	1.953	1.9555
S2	3.2ng/ml	1.308	1.3090
S2	3.2ng/ml	1.310	1.3090
S3	1.6ng/ml	0.698	0.7045
S3	1.6ng/ml	0.711	0.7045
S4	0.8ng/ml	0.397	0.3935
S4	0.8ng/ml	0.390	0.3935
S5	0.4ng/ml	0.209	0.2075
S5	0.4ng/ml	0.206	0.2075

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)