Summary:

Purpose

Our Quick Step Fish malondialdehyde (MDA) ELISA Kit is to assay MDA levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to MDA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for MDA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MDA and HRP conjugated MDA Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MDA. You can calculate the concentration of MDA in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0067FI is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.059	0.0560
Blank Well	0ng/ml	0.053	0.0560
S1	360ng/ml	1.975	1.9695
S1	360ng/ml	1.964	1.9695
S2	240ng/ml	1.388	1.3800
S2	240ng/ml	1.372	1.3800
S3	120ng/ml	0.705	0.6995
S3	120ng/ml	0.694	0.6995
S4	60ng/ml	0.383	0.3800
S4	60ng/ml	0.377	0.3800
S5	30ng/ml	0.197	0.2010
S5	30ng/ml	0.205	0.2010