Summary:

Purpose

Our Quick Step Horse Melatonin (MT) ELISA Kit is to assay MT levels in Horse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MT . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MT and HRP conjugated MT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MT . You can calculate the concentration of MT in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0041Ho is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.042	0.0485
Blank Well	0pg/ml	0.055	0.0485
S1	120pg/ml	1.998	1.9855
S1	120pg/ml	1.973	1.9855
S2	80pg/ml	1.318	1.3240
S2	80pg/ml	1.330	1.3240
S3	40pg/ml	0.688	0.6915
S3	40pg/ml	0.695	0.6915
S4	20pg/ml	0.393	0.3875
S4	20pg/ml	0.382	0.3875
S5	10pg/ml	0.201	0.1950
S5	10pg/ml	0.189	0.1950