Summary:

Purpose

Our Quick Step Hamster Apolipoprotein A1(APO-A1)

ELISA Kit

is to assay APO-A1

levels in Hamster serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to APO-A1

. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for APO-A1

is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain APO-A1

and HRP conjugated APO-A1

antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of APO-A1

. You can calculate the concentration of APO-A1

in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0023Ha is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/m	0.048	0.0465
Blank Well	0ng/m	0.045	0.0465
S1	18ng/m	1.973	1.9760
S1	18ng/m	1.979	1.9760
S2	12ng/m	1.363	1.3675
S2	12ng/m	1.372	1.3675
S3	6ng/m	0.725	0.7220
S3	6ng/m	0.719	0.7220
S4	3ng/m	0.388	0.3860
S4	3ng/m	0.384	0.3860
S5	1.5ng/m	0.187	0.1895
S5	1.5ng/m	0.192	0.1895