Summary:

Purpose

Our Quick Step Monkey Granulocyte Colony Stimulating Factor(G-CSF)ELISA Kit is to assay G-CSF levels in Monkey serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to G-CSF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for G-CSF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain G-CSF and HRP conjugated G-CSF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of G-CSF. You can calculate the concentration of G-CSF in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0020Mk is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
Blank Well	0pg/ml	0.053	0.0505
S1	180pg/ml	1.941	1.9370
S1	180pg/ml	1.933	1.9370
S2	120pg/ml	1.317	1.3255
S2	120pg/ml	1.334	1.3255
S3	60pg/ml	0.709	0.7200
S3	60pg/ml	0.731	0.7200
S4	30pg/ml	0.373	0.3645
S4	30pg/ml	0.356	0.3645
S5	15pg/ml	0.187	0.1900
S5	15pg/ml	0.193	0.1900