DESCRIPTION

ULBP3 (UL16-binding protein 3) is a member of the MHC class I-related molecule family, functioning as an activating ligand for the NKG2D receptor. It mediates natural killer cell cytotoxicity and plays roles in immune surveillance, tumor immunity, and viral immune evasion. The gene is located on chromosome 6q25.1 and is expressed in various tissues. Dysregulated ULBP3 expression has been implicated in autoimmune diseases and cancer, while viruses such as human cytomegalovirus target ULBP3 to escape immune detection.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	401.3	6.5	1.6
2	8	99.6	2.2	2.2
3	8	52.0	1.1	2.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	403.6	6.5	1.6
2	16	100.9	2.8	2.8
3	16	52.0	1.3	2.5

RECOVERY

The recovery of human ULBP3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	93	90-97
	1:4	96	89-105
Cell culture supernatant	1:4	100	96-102
	1:8	102	98-109

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	95	98
	Range (%)	72-118	95-102
1:8	Average% of Expected	100	113
	Range (%)	85-115	103-123
1:16	Average% of Expected	87	112
	Range (%)	76-98	109-116

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human ULBP3 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)