DESCRIPTION

Ephrin A1 is also named LERK1 and TNFAIP4. It belongs to the ephrin family. Ephrin A1 is the cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. It binds promiscuously Eph receptors residing on adjacent cells, leading to contactdependent bidirectional signaling into neighboring cells. Ephrin A1 is involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2. Ephrin A1 acts as a ligand for EPHA3, inhibiting epithelial-tomesenchymal transition of cardiac cells and playing a role in heart development.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,611.2	19.6	1.2
2	8	401.6	13.6	3.4
3	8	198.9	6.7	3.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,598.1	44.0	2.8
2	16	397.7	14.0	3.5
3	16	200.1	10.1	5.0

RECOVERY

The recovery of human Ephrin A1 spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	75	72-76
	1:4	87	81-92

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of human Ephrin A1 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:2	Average% of Expected	73
	Range (%)	70-77
1:4	Average% of Expected	87
	Range (%)	80-93
1:8	Average% of Expected	92
	Range (%)	83-101
1:16	Average% of Expected	106
	Range (%)	97-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Ephrin A1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)