DESCRIPTION

IL-17RB is a transmembrane protein that belongs to the IL-17 receptor family. It is expressed in various endocrine tissues and epithelial cells in different organs such as kidney, liver, and mucosal tissues. The IL-17B/IL-17RB pathway is considered as a signaling cascade that promotes cancer cell survival, proliferation and migration. The pro-tumor functions associated with the IL 17B/IL-17RB pathway are diverse and complex because they involve mechanisms that act directly on tumor cells, and also indirect mechanisms that lead to tumor microenvironment remodeling.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,282.0	15.9	1.2
2	8	317.4	7.2	2.3
3	8	154.3	4.1	2.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,302.2	26.8	2.1
2	16	318.6	5.6	1.8
3	16	156.3	3.7	2.4

RECOVERY

The recovery of mouse IL-17RB spiked to three different levels throughout the range of the assay in tissue lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Tissue lysate	1:4	114	106-123
	1:8	110	105-118

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Tissue lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	99
	Range (%)	95-103
1:8	Average% of Expected	102
	Range (%)	101-103
1:16	Average% of Expected	104
	Range (%)	103-104

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse IL-17RB One-Step ELISA Kit produced at Sunlong Medical™.